7DA1
Crystal structure of the chicken MHF complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-11 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.432, 59.432, 220.949 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.460 - 2.010 |
| R-factor | 0.1987 |
| Rwork | 0.197 |
| R-free | 0.22480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b0b |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.909 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.460 | 2.082 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.103 | 0.772 |
| Rmeas | 0.108 | 0.802 |
| Rpim | 0.030 | 0.217 |
| Number of reflections | 27614 | 2661 |
| <I/σ(I)> | 22.3 | 3 |
| Completeness [%] | 99.8 | |
| Redundancy | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M MOPS-HEPES pH 7.0, 0.1 M Carboxylic acids mix, 15% MPD, 20% PEG3350 |
