7DA0
High-resolution crystal structure of the chicken MHF complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-18 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 50.566, 69.071, 48.878 |
Unit cell angles | 90.00, 103.67, 90.00 |
Refinement procedure
Resolution | 34.010 - 1.250 |
R-factor | 0.1936 |
Rwork | 0.193 |
R-free | 0.21230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3b0b |
RMSD bond length | 0.009 |
RMSD bond angle | 0.975 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.010 | 1.295 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.006 | 0.851 |
Rmeas | 0.066 | 0.999 |
Rpim | 0.034 | 0.520 |
Number of reflections | 43638 | 4340 |
<I/σ(I)> | 36.64 | 2.17 |
Completeness [%] | 96.7 | 96.21 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris-Bicine pH 8.5, 0.1 M Carboxylic acids mix, 12.5% MPD, 12.5% PEG 1000, 12.5% PEG 3350 |