7DA0
High-resolution crystal structure of the chicken MHF complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 50.566, 69.071, 48.878 |
| Unit cell angles | 90.00, 103.67, 90.00 |
Refinement procedure
| Resolution | 34.010 - 1.250 |
| R-factor | 0.1936 |
| Rwork | 0.193 |
| R-free | 0.21230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b0b |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.975 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.010 | 1.295 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.006 | 0.851 |
| Rmeas | 0.066 | 0.999 |
| Rpim | 0.034 | 0.520 |
| Number of reflections | 43638 | 4340 |
| <I/σ(I)> | 36.64 | 2.17 |
| Completeness [%] | 96.7 | 96.21 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris-Bicine pH 8.5, 0.1 M Carboxylic acids mix, 12.5% MPD, 12.5% PEG 1000, 12.5% PEG 3350 |
