7D9M
grass carp interleukin-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-10-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 83.090, 83.090, 302.931 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.420 - 2.660 |
| R-factor | 0.3779 |
| Rwork | 0.377 |
| R-free | 0.41170 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.175 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 10.000 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.097 | |
| Number of reflections | 17766 | 8666 |
| <I/σ(I)> | 15.3 | |
| Completeness [%] | 93.1 | |
| Redundancy | 5.2 | |
| CC(1/2) | 0.738 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 1.4 M Sodium phosphate monobasic monohydrate, Potassium Phosphate dibasic (pH 8.2) |
