7D9J
SpdH Spermidine dehydrogenase Y443A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97737 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.051, 85.501, 101.109 |
| Unit cell angles | 90.00, 98.46, 90.00 |
Refinement procedure
| Resolution | 34.960 - 2.190 |
| R-factor | 0.1494 |
| Rwork | 0.147 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7d9f |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.906 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rpim | 0.040 | 0.144 |
| Number of reflections | 59220 | 2898 |
| <I/σ(I)> | 18.2 | 5 |
| Completeness [%] | 99.2 | 97.7 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 0.1M Bis Tris pH6.5, 20% w/v polyethylene glycol (PEG) 3350 |
