7D9G
SpdH Spermidine dehydrogenase native structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97700 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.399, 85.731, 100.654 |
| Unit cell angles | 90.00, 98.45, 90.00 |
Refinement procedure
| Resolution | 49.780 - 2.400 |
| R-factor | 0.1859 |
| Rwork | 0.183 |
| R-free | 0.24650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7d9f |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.988 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rpim | 0.077 | 0.241 |
| Number of reflections | 45554 | 2136 |
| <I/σ(I)> | 9.7 | 2.8 |
| Completeness [%] | 99.5 | 94.6 |
| Redundancy | 6.5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 0.1M Bis tris pH 6.5, 20% w/v polyethylene glycol 3350 |
