7D9F
SpdH Spermidine dehydrogenase SeMet Structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97778 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.197, 85.586, 99.624 |
| Unit cell angles | 90.00, 98.33, 90.00 |
Refinement procedure
| Resolution | 39.250 - 1.850 |
| R-factor | 0.1511 |
| Rwork | 0.150 |
| R-free | 0.19030 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.836 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rpim | 0.057 | 0.345 |
| Number of reflections | 95318 | 4659 |
| <I/σ(I)> | 13.9 | 2.3 |
| Completeness [%] | 97.7 | 95.8 |
| Redundancy | 6.4 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1M Bis Tris pH6.5, 20% w/v polyethylene glycol (PEG) 3350 |
