7D9C
Alpha-glucosidase from Weissella cibaria BBK-1 bound with maltose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2019-03-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 115.636, 115.636, 106.163 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.760 - 1.360 |
R-factor | 0.1403 |
Rwork | 0.140 |
R-free | 0.15340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7d9b |
RMSD bond length | 0.015 |
RMSD bond angle | 1.430 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (7.0.069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.410 |
High resolution limit [Å] | 1.360 | 1.360 |
Rmeas | 0.108 | 0.946 |
Rpim | 0.041 | 0.358 |
Number of reflections | 152899 | 15113 |
<I/σ(I)> | 48.34 | |
Completeness [%] | 99.9 | |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Ammonium sulfate, dioxane, MES |