7D98
Crystal structure of full-length CbnR complexed with the target DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2016-12-13 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.9000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 105.819, 105.819, 602.516 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.600 |
| R-factor | 0.237 |
| Rwork | 0.234 |
| R-free | 0.28610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iz1 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.706 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2-3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.890 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rpim | 0.061 | 0.458 |
| Number of reflections | 23984 | 4793 |
| <I/σ(I)> | 12 | 2.1 |
| Completeness [%] | 98.5 | |
| Redundancy | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20%(w/v) PEG 3350, 0.5%(w/v) n-Octyl-pyranoside, 4%(v/v) Tacsimate |
