7D97
Crystal structure of N109P mutant of GATase subunit of Methanocaldococcus jannaschii GMP synthetase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 2020-02-17 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | P 1 |
Unit cell lengths | 35.500, 68.050, 75.350 |
Unit cell angles | 90.95, 90.47, 91.45 |
Refinement procedure
Resolution | 35.490 - 1.890 |
R-factor | 0.207 |
Rwork | 0.204 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7d40 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.624 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.490 | 2.030 |
High resolution limit [Å] | 1.890 | 1.890 |
Number of reflections | 48293 | 5717 |
<I/σ(I)> | 1.28 | |
Completeness [%] | 93.7 | |
Redundancy | 2.1 | |
CC(1/2) | 0.861 | 0.702 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.6 | 277 | 100 mM Sodium acetate, pH 5.6, 20 % PEG 3350 |