7D8V
Crystal Structure of A Kinesin-3 KIF13B mutant-T192Y
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 87.510, 87.510, 97.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.920 - 2.300 |
R-factor | 0.1848 |
Rwork | 0.182 |
R-free | 0.24070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6a1z |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.560 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.143 | 1.059 |
Number of reflections | 19638 | 961 |
<I/σ(I)> | 10.4 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.8 | 9.7 |
CC(1/2) | 0.997 | 0.725 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 289 | 0.1M Bis-Tris propane, 30% (w/v) PEG 20000 |