7D8S
MITF bHLHLZ apo structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 |
Unit cell lengths | 56.005, 62.306, 65.307 |
Unit cell angles | 90.12, 72.74, 86.39 |
Refinement procedure
Resolution | 38.942 - 2.280 |
R-factor | 0.2334 |
Rwork | 0.231 |
R-free | 0.27050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6KTD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.048 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.360 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.035 | 0.305 |
Number of reflections | 34574 | 3686 |
<I/σ(I)> | 37 | |
Completeness [%] | 96.5 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | Sodium acetate pH 4.5, Ammonium sulfate |