7D8R
MITF HLHLZ structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.910, 78.120, 80.590 |
| Unit cell angles | 93.18, 105.03, 105.62 |
Refinement procedure
| Resolution | 38.693 - 3.000 |
| R-factor | 0.2866 |
| Rwork | 0.285 |
| R-free | 0.31820 |
| Structure solution method | SAD |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 77.124 | 38.693 | 3.160 |
| High resolution limit [Å] | 3.000 | 9.490 | 3.000 |
| Rmerge | 0.037 | 0.586 | |
| Rmeas | 0.061 | 0.043 | 0.678 |
| Rpim | 0.031 | 0.023 | 0.341 |
| Total number of observations | 72600 | 2176 | 10427 |
| Number of reflections | 18512 | 582 | 2651 |
| <I/σ(I)> | 12.7 | 28.7 | 2.4 |
| Completeness [%] | 98.3 | 95.5 | 98 |
| Redundancy | 3.9 | 3.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | HEPES, KCl, PO/OH |
