7D8M
Crystal structure of DyP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 65 |
| Unit cell lengths | 118.959, 118.959, 65.347 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.399 - 2.000 |
| R-factor | 0.1709 |
| Rwork | 0.169 |
| R-free | 0.20870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3afv |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.420 | 2.000 |
| Rmerge | 0.125 | 0.069 | 0.815 |
| Rmeas | 0.132 | 0.072 | 0.872 |
| Rpim | 0.041 | 0.022 | 0.306 |
| Total number of observations | 360800 | ||
| Number of reflections | 35763 | 1836 | 1776 |
| <I/σ(I)> | 2.9 | ||
| Completeness [%] | 99.8 | 98.7 | 99.7 |
| Redundancy | 10.1 | 10.2 | 7.9 |
| CC(1/2) | 0.996 | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Ammonium acetate, 0.1M Sodium acetate trihydrate, 30% w/v PEG 4000, pH 4.9 |
