7D8D
The crystal structure of ScNTM1 in complex with SAH and Rps25a hexapeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-01-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97891 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.692, 52.014, 127.723 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.210 - 1.050 |
| R-factor | 0.1187 |
| Rwork | 0.118 |
| R-free | 0.13570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ex4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.110 |
| Data reduction software | HKL-2000 (1.18.2_3874, 1.10.1_2155) |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.020 |
| High resolution limit [Å] | 0.999 | 1.000 |
| Rmeas | 0.094 | |
| Rpim | 0.031 | |
| Number of reflections | 130264 | 3404 |
| <I/σ(I)> | 38.8 | |
| Completeness [%] | 93.0 | 49 |
| Redundancy | 8.6 | |
| CC(1/2) | 0.998 | 0.032 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2M magnesium chloride hexahydrate,0.1M Bis-Tris(pH 6.5) and 25% PEG3350 |
