7D8D
The crystal structure of ScNTM1 in complex with SAH and Rps25a hexapeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-01-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97891 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.692, 52.014, 127.723 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.210 - 1.050 |
R-factor | 0.1187 |
Rwork | 0.118 |
R-free | 0.13570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ex4 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.110 |
Data reduction software | HKL-2000 (1.18.2_3874, 1.10.1_2155) |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.020 |
High resolution limit [Å] | 0.999 | 1.000 |
Rmeas | 0.094 | |
Rpim | 0.031 | |
Number of reflections | 130264 | 3404 |
<I/σ(I)> | 38.8 | |
Completeness [%] | 93.0 | 49 |
Redundancy | 8.6 | |
CC(1/2) | 0.998 | 0.032 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.2M magnesium chloride hexahydrate,0.1M Bis-Tris(pH 6.5) and 25% PEG3350 |