7D84
34-fold symmetry Salmonella S ring formed by full-length FliF
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON II (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.7 |
| Number of Particle | 339861 |
| Software | Gautomatch,RELION,cryoSPARC,PHENIX,EPU,Gctf,UCSF Chimera,Coot |
| Symmetry | C34 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
