7D7Q

Crystal structure of the transmembrane domain and linker region of Salpingoeca rosetta rhodopsin phosphodiesterase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	76.300, 136.530, 206.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.400 - 3.500
R-factor	0.2686
Rwork	0.266
R-free	0.31440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cj3
RMSD bond length	0.002
RMSD bond angle	0.635
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.400	3.710
High resolution limit [Å]	3.500	3.500
Rmeas	0.673	2.868
Number of reflections	13712	2147
<I/σ(I)>	3.9
Completeness [%]	98.1
Redundancy	6.55
CC(1/2)	0.934	0.393

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5	293	25% (w/v) PEG 500DME, 100mM Na-citrate, pH 5.0, 100mM NaK-tartrate