7D6T
X-ray structure of Clostridium perfringens sortase C with the C-terminal cell wall sorting motif.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-10-09 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.230, 62.690, 77.690 |
| Unit cell angles | 90.00, 102.58, 90.00 |
Refinement procedure
| Resolution | 19.020 - 1.680 |
| R-factor | 0.15873 |
| Rwork | 0.155 |
| R-free | 0.22033 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ixz |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.535 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.020 | 1.720 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Number of reflections | 43526 | 3217 |
| <I/σ(I)> | 12.56 | |
| Completeness [%] | 97.6 | |
| Redundancy | 2.4 | |
| CC(1/2) | 0.998 | 0.839 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 30% (w/v) PEG 4000, 200mM LiSO4, 100mM Tris-HCl, pH 7.5 |
