7D6I
A neutralizing MAb targeting receptor-binding-domain of SARS-CoV-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-10 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 150.605, 150.605, 121.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.300 - 3.410 |
| R-factor | 0.2271 |
| Rwork | 0.226 |
| R-free | 0.25510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lzg |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.803 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 3.620 |
| High resolution limit [Å] | 3.410 | 3.410 |
| Number of reflections | 15877 | 800 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 36.1 | |
| CC(1/2) | 0.993 | 0.733 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 50 mM Calcium chloride dihydrate, 100 mM BIS-TRIS pH 6.5, 30% v/v Polyethylene glycol and monomethyl ether 550 |
