7D66
Crystal structure of retroviral protease-like domain of Ddi1 from Toxoplasma gondii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-10 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.99 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 184.601, 184.601, 184.338 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.266 - 2.126 |
Rwork | 0.192 |
R-free | 0.24750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2i1a |
RMSD bond length | 0.015 |
RMSD bond angle | 1.833 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.270 | 2.182 |
High resolution limit [Å] | 2.120 | 2.126 |
Number of reflections | 176969 | 8844 |
<I/σ(I)> | 13.9 | |
Completeness [%] | 100.0 | |
Redundancy | 8.6 | |
CC(1/2) | 0.990 | 0.365 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 293 | 2 M ammonium citrate tribasic, 30 % 2-propanol |