7D5P
Structure of NorC transporter in an outward-open conformation in complex with a single-chain Indian camelid antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.212, 139.875, 118.034 |
| Unit cell angles | 90.00, 106.03, 90.00 |
Refinement procedure
| Resolution | 48.960 - 3.650 |
| R-factor | 0.29839 |
| Rwork | 0.297 |
| R-free | 0.31763 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.213 |
| Data reduction software | XDS |
| Data scaling software | ADDREF |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.180 | 3.900 |
| High resolution limit [Å] | 3.650 | 3.650 |
| Rmerge | 0.196 | 4.140 |
| Rpim | 0.048 | 1.050 |
| Number of reflections | 24592 | 4248 |
| <I/σ(I)> | 8 | 1 |
| Completeness [%] | 99.1 | 95.2 |
| Redundancy | 17.5 | 16.1 |
| CC(1/2) | 0.997 | 0.563 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M HEPES, pH 6.0, 50 mM NaCl, 35.7% PEG 600, 57.1 mM CaCl2, 10 mM YtCl2, 6.66 mM CHAPSO |
