7D4D
Structure of L-lysine oxidase precursor in complex with L-lysine (1.24M)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-26 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 94.310, 127.960, 94.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 75.918 - 2.290 |
R-factor | 0.1717 |
Rwork | 0.169 |
R-free | 0.22070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3x0v |
RMSD bond length | 0.008 |
RMSD bond angle | 0.925 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.400 | 2.370 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.074 | 0.391 |
Rpim | 0.056 | 0.304 |
Number of reflections | 25914 | 2533 |
<I/σ(I)> | 12.9 | 3.2 |
Completeness [%] | 99.4 | 99.6 |
Redundancy | 4.1 | 4 |
CC(1/2) | 0.996 | 0.861 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M Tris-HCl pH8.0 and 2.1 M Potassium Sulfate |