7D48
apo-form cyclic trinucleotide synthase CdnD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-02-22 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 52.924, 52.924, 344.361 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.010 - 2.150 |
R-factor | 0.185 |
Rwork | 0.182 |
R-free | 0.22800 |
Structure solution method | MAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.593 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.220 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.096 | 0.403 |
Number of reflections | 27647 | |
<I/σ(I)> | 17 | 2.7 |
Completeness [%] | 98.9 | 97.5 |
Redundancy | 8.7 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 8% v/v Tacsimate, pH 6.0, 20% w/v PEG 3350 |