7D3Y

Crystal structure of the osPHR2-osSPX2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	147.812, 147.812, 143.287
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.380 - 3.110
R-factor	0.2219
Rwork	0.220
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7d3t
RMSD bond length	0.007
RMSD bond angle	0.563
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.380	3.280
High resolution limit [Å]	3.110	3.110
Rmerge	0.049	0.844
Rmeas	0.051	0.865
Rpim	0.012	0.188
Number of reflections	32993	4743
<I/σ(I)>	35.4	4.7
Completeness [%]	99.9
Redundancy	19.8
CC(1/2)	0.999	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	Ammonium sulfate, Pentaerythritol ethoxylate, Tacsimate