7D3J

Crystal structure of the Cas12i1 R-loop complex after target DNA cleavage

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	129.801, 141.974, 208.296
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.730 - 2.450
R-factor	0.222
Rwork	0.220
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7d2l
Data reduction software	xia2
Data scaling software	Aimless (0.7.1)
Phasing software	PHENIX (1.13-2998)
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.730	2.510
High resolution limit [Å]	2.450	2.450
Rmerge	0.083	0.767
Number of reflections	70800	4504
<I/σ(I)>	17.6	3.4
Completeness [%]	100.0
Redundancy	13.4
CC(1/2)	0.999	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M sodium citrate (pH 5.4), 15% (w/v) Polyethylene glycol 3350, 0.2 M lithium chloride