7D39
FLR-apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97850 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.870, 67.870, 194.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.284 - 2.198 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.21070 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.899 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.198 | 2.198 |
| Number of reflections | 21222 | 21222 |
| <I/σ(I)> | 34.5 | |
| Completeness [%] | 99.6 | |
| Redundancy | 22 | |
| CC(1/2) | 0.788 | 0.788 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M magnesium formate dihydrate and 15 w/v polyethylene glycol 3350 |
