7D38

flavone reductase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 43 21 2
Unit cell lengths	66.365, 66.365, 194.057
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.908 - 2.649
R-factor	0.1961
Rwork	0.190
R-free	0.24780
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.013
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.740
High resolution limit [Å]	2.649	2.650
Number of reflections	20566	11637
<I/σ(I)>	30
Completeness [%]	99.7
Redundancy	21.8
CC(1/2)	0.998	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M magnesium formate dihydrate and 15 w/v polyethylene glycol 3350