7D34

AtClpS1-peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	173
Detector technology	IMAGE PLATE
Collection date	2018-10-10
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54178
Spacegroup name	P 21 21 2
Unit cell lengths	81.056, 38.005, 61.411
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.410 - 2.007
R-factor	0.2769
Rwork	0.274
R-free	0.29870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o2x
RMSD bond length	0.009
RMSD bond angle	1.025
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.040
High resolution limit [Å]	2.000	2.000
Rpim	0.028	0.168
Number of reflections	13209	632
<I/σ(I)>	48.8
Completeness [%]	99.8
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M sodium acetate trihydrate pH 4.6 (Hampton Research, HR2-731), 2 M NaCl (Hampton Research, HR2-637)