7D2Y

complex of two RRM domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 62 2 2
Unit cell lengths	116.961, 116.961, 140.168
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.361 - 2.680
R-factor	0.1989
Rwork	0.195
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7d1l
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.780
High resolution limit [Å]	2.680	2.680
Rmerge		1.161
Number of reflections	27391	1593
<I/σ(I)>	0.039
Completeness [%]	100.0
Redundancy	37.7
CC(1/2)	1.000	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Tris pH 8.5, 2.0M Ammonium Sulfate