7D2U

Crystal structure of Rsu1/PINCH1_LIM45C complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	I 2 2 2
Unit cell lengths	51.354, 144.397, 185.043
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.534 - 3.150
R-factor	0.1929
Rwork	0.192
R-free	0.21620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7d2s
RMSD bond length	0.002
RMSD bond angle	0.547
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.200
High resolution limit [Å]	3.150	8.540	3.150
Rmerge	0.131	0.097	0.967
Rmeas	0.137	0.102	1.009
Rpim	0.039	0.031	0.284
Number of reflections	12561	699	629
<I/σ(I)>	4.8
Completeness [%]	99.9	99.3	100
Redundancy	12.9	10.3	12.4
CC(1/2)		0.995	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1M Tris8.0, 8% w/v PEG8000