7D2S

Crystal structure of Rsu1/PINCH1_LIM5C complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	I 4
Unit cell lengths	124.596, 124.596, 50.518
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.653
R-factor	0.1669
Rwork	0.166
R-free	0.18450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u06
RMSD bond length	0.006
RMSD bond angle	1.012
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.680
High resolution limit [Å]	1.650	4.480	1.650
Rmerge	0.089	0.063	0.931
Rmeas	0.093	0.066	0.969
Rpim	0.025	0.018	0.269
Total number of observations	623057
Number of reflections	46579	2416	2321
<I/σ(I)>	6.9
Completeness [%]	100.0	100	100
Redundancy	13.4	13.3	12.9
CC(1/2)		0.997	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1M Bicine pH8.5, 20% w/v PEG10000