7D2Q

Crystal structure of MazE-MazF (Form-I) from Deinococcus radiodurans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	193
Detector technology	CCD
Collection date	2017-10-22
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	45.486, 74.083, 136.852
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.300 - 1.990
R-factor	0.1805
Rwork	0.178
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7d28
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	1.990	5.430	2.000
Rmerge	0.114	0.063	0.778
Rmeas	0.119	0.067	0.809
Rpim	0.034	0.021	0.221
Number of reflections	32418	1780	1588
<I/σ(I)>	5.5
Completeness [%]	99.9	99	100
Redundancy	12.8	10.3	13.2
CC(1/2)		0.995	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM citric acid pH 3.5 and 25% PEG 3,350