7D2P
Crystal structure of MazE-MazF (Form-II) from Deinococcus radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2017-10-02 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97935 |
Spacegroup name | P 1 |
Unit cell lengths | 39.824, 42.327, 64.998 |
Unit cell angles | 96.51, 97.82, 86.71 |
Refinement procedure
Resolution | 29.400 - 2.070 |
R-factor | 0.173 |
Rwork | 0.168 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7d28 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.545 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.063 | 0.034 | 0.365 |
Rmeas | 0.074 | 0.041 | 0.424 |
Rpim | 0.038 | 0.022 | 0.215 |
Total number of observations | 104692 | ||
Number of reflections | 28134 | 1329 | 1411 |
<I/σ(I)> | 8.4 | ||
Completeness [%] | 97.2 | 91.9 | 96.2 |
Redundancy | 3.7 | 3.3 | 3.9 |
CC(1/2) | 0.992 | 0.902 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M succinic acid pH 7.0 and 12% w/v PEG 3,350 |