7D2N
Crystal structure of MazE-MazF (Form-III) from Deinococcus radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2018-09-15 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97940 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 138.606, 56.215, 61.822 |
Unit cell angles | 90.00, 107.31, 90.00 |
Refinement procedure
Resolution | 33.080 - 1.600 |
R-factor | 0.2323 |
Rwork | 0.231 |
R-free | 0.24770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7d28 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.630 |
High resolution limit [Å] | 1.600 | 4.340 | 1.600 |
Rmerge | 0.085 | 0.053 | 0.872 |
Rmeas | 0.099 | 0.062 | 1.010 |
Rpim | 0.050 | 0.032 | 0.509 |
Total number of observations | 446024 | ||
Number of reflections | 58095 | 5704 | 5621 |
<I/σ(I)> | 7.3 | ||
Completeness [%] | 97.0 | 97.5 | 94.8 |
Redundancy | 3.9 | 3.9 | 3.9 |
CC(1/2) | 0.996 | 0.819 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Sodium thiocyanate, 20% PEG 3,350, pH 6.9 |