7D2M

Crystal structure of MazF (Form-II) from Deinococcus radiodurans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	193
Detector technology	CCD
Collection date	2019-10-21
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 43 21 2
Unit cell lengths	72.451, 72.451, 112.517
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.910 - 1.790
R-factor	0.1576
Rwork	0.156
R-free	0.18050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7d28
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	23.000	23.000	1.830
High resolution limit [Å]	1.790	4.870	1.800
Rmerge	0.181	0.097	0.407
Rmeas	0.186	0.100	0.418
Rpim	0.038	0.022	0.094
Number of reflections	28465	1577	1356
<I/σ(I)>	8.7
Completeness [%]	99.4	98.3	99.1
Redundancy	20	20.8	17.9
CC(1/2)		0.992	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM Lithium Sulfate monohydrate, Tris HCl pH 8.5 and 30% PEG 4,000