7D2H

Tetrameric coiled-coil structure of liprin-alpha2_H2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	32.879, 101.907, 37.445
Unit cell angles	90.00, 96.41, 90.00

Refinement procedure

Resolution	30.051 - 2.200
R-factor	0.2313
Rwork	0.229
R-free	0.28150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7d2g
RMSD bond length	0.001
RMSD bond angle	0.342
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.080	0.042	0.847
Rmeas	0.092	0.048	0.980
Rpim	0.044	0.023	0.483
Total number of observations	43572
Number of reflections	11895	580	599
<I/σ(I)>	9.5
Completeness [%]	93.1	87.1	94.3
Redundancy	3.7	3.7	3.6
CC(1/2)		0.998	0.753

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	20% v/v 2-Propanol, 0.1 M Tris pH 8.0, 5% w/v Polyethylene glycol 8,000