7D2G

Coiled-coil structure of liprin-alpha2_H2delC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-01-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	126.057, 33.410, 73.631
Unit cell angles	90.00, 111.25, 90.00

Refinement procedure

Resolution	50.000 - 1.700
R-factor	0.2375
Rwork	0.236
R-free	0.26250
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.369
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.760
High resolution limit [Å]	1.700	3.660	1.700
Rmerge	0.132	0.094	1.312
Rmeas	0.138	0.099	1.376
Rpim	0.041	0.031	0.405
Number of reflections	31730	3116	3006
<I/σ(I)>	8.3
Completeness [%]	98.0	92.4	92.4
Redundancy	12.5	10.7	9.6
CC(1/2)		0.991	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M Sodium thiocyanate, 20% w/v Polyethylene glycol 3,350