7D2E
Tetrameric coiled-coil structure of liprin-alpha2_H3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.307, 71.293, 53.473 |
| Unit cell angles | 90.00, 103.69, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.2409 |
| Rwork | 0.239 |
| R-free | 0.26850 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.758 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
| Rmerge | 0.037 | 0.022 | 0.757 |
| Rmeas | 0.043 | 0.026 | 0.886 |
| Rpim | 0.022 | 0.013 | 0.457 |
| Number of reflections | 30192 | 1259 | 1528 |
| <I/σ(I)> | 15.6 | ||
| Completeness [%] | 98.4 | 79 | 99.5 |
| Redundancy | 3.7 | 3.6 | 3.7 |
| CC(1/2) | 0.997 | 0.744 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 10% w/v Polyethylene glycol 6,000 |
