7D29
CBM32 of AlyQ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-05-04 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.447, 55.670, 58.496 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.252 - 1.700 |
| Rwork | 0.155 |
| R-free | 0.18930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xnr |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.560 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.022 | 0.066 |
| Rmeas | 0.027 | 0.081 |
| Number of reflections | 14744 | 969 |
| <I/σ(I)> | 33.3 | 13.2 |
| Completeness [%] | 91.2 | 61 |
| Redundancy | 3.15 | 2.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M sodium HEPES, 20% PEG 10000 |
