7D28
Crystal structure of MazF (Form-I) from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2017-07-21 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97933 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.542, 74.976, 120.103 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.820 - 1.500 |
| R-factor | 0.1723 |
| Rwork | 0.171 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ck9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.874 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
| Rmerge | 0.078 | 0.041 | 0.908 |
| Rmeas | 0.081 | 0.043 | 0.941 |
| Rpim | 0.021 | 0.012 | 0.246 |
| Total number of observations | 1021009 | ||
| Number of reflections | 71103 | 3806 | 3520 |
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 100.0 | 99.2 | 100 |
| Redundancy | 14.4 | 12.6 | 14.4 |
| CC(1/2) | 0.999 | 0.922 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 5000 MME, 100 mM MES pH 6.5 and 200 mM Ammonium sulfate |
