7D27

Structure of UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	104.15
Detector technology	PIXEL
Collection date	2020-05-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97949
Spacegroup name	P 42 21 2
Unit cell lengths	119.960, 119.960, 116.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.001 - 2.480
Rwork	0.228
R-free	0.25980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bub
RMSD bond length	0.015
RMSD bond angle	1.776
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.180	2.570
High resolution limit [Å]	2.480	2.480
Rpim	0.023	0.424
Number of reflections	30793	3022
<I/σ(I)>	20.4
Completeness [%]	100.0
Redundancy	17.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	21%(v/v) 2-propanl, 30% (v/v) Glyceol 0.07M sodium cacodylate trihydrate pH 6.5, 0.14M Sodium citrate tribasic dihydrate