Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.450, 99.020, 59.583 |
| Unit cell angles | 90.00, 108.54, 90.00 |
Refinement procedure
| Resolution | 49.560 - 1.350 |
| R-factor | 0.1187 |
| Rwork | 0.117 |
| R-free | 0.15700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f9f |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.561 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.370 |
| High resolution limit [Å] | 1.350 | 3.660 | 1.350 |
| Rmerge | 0.047 | 0.019 | 1.089 |
| Rmeas | 0.051 | 0.020 | 1.231 |
| Rpim | 0.020 | 0.008 | 0.553 |
| Total number of observations | 843693 | ||
| Number of reflections | 131802 | 6800 | 5755 |
| <I/σ(I)> | 11.4 | ||
| Completeness [%] | 98.7 | 99.9 | 86.8 |
| Redundancy | 6.4 | 6.7 | 4.1 |
| CC(1/2) | 0.999 | 0.517 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 291 | 10mM Tris, 1mM EDTA, 1mM DTT |
