7D1B
Crystal structure of Fimbriiglobus ruber glutaminyl cyclase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-08-01 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.206, 73.436, 87.427 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.967 - 1.240 |
R-factor | 0.1113 |
Rwork | 0.111 |
R-free | 0.12540 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.280 |
High resolution limit [Å] | 1.240 | 1.240 |
Rmerge | 0.041 | 0.348 |
Number of reflections | 102036 | 9674 |
<I/σ(I)> | 41.8 | 5.1 |
Completeness [%] | 97.4 | 93.6 |
Redundancy | 7.4 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 20% (w/v) PEG 8000, 0.1 M Tris-HCl, pH 8.5, 0.1 M MgCl2, 20% (v/v) PEG 400 |