7D0W

Crystal structure of Hen Egg White Lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	76.790, 76.790, 37.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.300 - 1.800
R-factor	0.1801
Rwork	0.178
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dpx
RMSD bond length	0.019
RMSD bond angle	1.882
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	76.790	38.395	1.900
High resolution limit [Å]	1.800	5.690	1.800
Rmerge		0.091	0.232
Rmeas	0.125	0.106	0.279
Rpim	0.065	0.053	0.151
Total number of observations	71152	2663	9801
Number of reflections	20691	693	2981
<I/σ(I)>	8.2	12.4	4.4
Completeness [%]	99.8	99.6	99.9
Redundancy	3.4	3.8	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298.15	PEG 3000, TRIS pH 5.6