7D0Q
Crystal structure of human HBO1-BRPF2 in complex with butyryl-coenzyme A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97892 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.871, 39.599, 87.900 |
| Unit cell angles | 90.00, 122.89, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.210 |
| R-factor | 0.1889 |
| Rwork | 0.186 |
| R-free | 0.24350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gk9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.212 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.560 |
| Number of reflections | 18377 | 1836 |
| <I/σ(I)> | 28.6 | 2.4 |
| Completeness [%] | 99.3 | |
| Redundancy | 6.6 | |
| CC(1/2) | 1.000 | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 M Tris, pH 8.5, 20% w/v PEG 6000 |
