7D0O

Crystal structure of human HBO1-BRPF2 in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-16
Detector	PSI PILATUS 6M
Wavelength(s)	0.97892
Spacegroup name	C 1 2 1
Unit cell lengths	127.604, 38.372, 88.075
Unit cell angles	90.00, 122.60, 90.00

Refinement procedure

Resolution	50.010 - 2.510
R-factor	0.2478
Rwork	0.246
R-free	0.29010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gk9
RMSD bond length	0.007
RMSD bond angle	1.243
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.010	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	11980	912
<I/σ(I)>	25.9	2.2
Completeness [%]	95.0	73.8
Redundancy	6.1	4.8
CC(1/2)	0.970	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	Tacsimate Tris-HCl, PEG 3350