7D0E

Crystal structure of FIP200 Claw/p-CCPG1 FIR2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97876
Spacegroup name	P 43 21 2
Unit cell lengths	51.438, 51.438, 86.151
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.070 - 1.400
Rwork	0.187
R-free	0.20680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dce
RMSD bond length	0.016
RMSD bond angle	1.481
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.037
Rpim		0.174
Number of reflections	23548	1143
<I/σ(I)>	98.9
Completeness [%]	99.8
Redundancy	25.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	KCl, Pentaerythritol ethoxylate (15/4 EO/OH), MES