7D04
Lysozyme structure SS3 from SS mode
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | PAL-XFEL BEAMLINE NCI |
Synchrotron site | PAL-XFEL |
Beamline | NCI |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2019-11-28 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.2782 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.895, 78.895, 37.811 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.890 - 1.700 |
R-factor | 0.2147 |
Rwork | 0.213 |
R-free | 0.24820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vds |
RMSD bond length | 0.007 |
RMSD bond angle | 1.050 |
Data reduction software | CrystFEL (V0.9.1) |
Data scaling software | CrystFEL (V0.9.1) |
Phasing software | PHENIX (V1.18.2_3874) |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.890 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 25173 | 2532 |
<I/σ(I)> | 3.52 | 1.56 |
Completeness [%] | 100.0 | |
Redundancy | 140.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 293 | 100 mM sodium acetate (pH 4.0), 6% (w/v) polyethylene glycol 8,000, and 3.5 M NaCl |