7CZO

N-terminal domain of HipA toxin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	293
Detector technology	CCD
Collection date	2019-11-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 41 21 2
Unit cell lengths	66.252, 66.252, 103.622
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.700
Rwork	0.242
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wiu
RMSD bond length	0.015
RMSD bond angle	1.902
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Number of reflections	6880	345
<I/σ(I)>	27.9
Completeness [%]	99.9
Redundancy	9.6
CC(1/2)	0.998	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1 M ammonium sulfate, 0.1 M HEPES, 0.5% (w/v) PEG 8000