7CZ0

Crystal structure of a thermostable green fluorescent protein (TGP) with a synthetic nanobody (Sb92)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-12
Detector	PSI PILATUS 6M
Wavelength(s)	0.97930
Spacegroup name	P 21 21 2
Unit cell lengths	95.245, 130.154, 171.453
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.620 - 2.770
R-factor	0.2042
Rwork	0.202
R-free	0.24060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lz2
RMSD bond length	0.004
RMSD bond angle	0.716
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.620	2.850
High resolution limit [Å]	2.770	2.770
Rmerge	0.148	1.147
Rpim	0.062	0.651
Number of reflections	54942	4418
<I/σ(I)>	9.2	1.7
Completeness [%]	99.9	99.9
Redundancy	6.7	7
CC(1/2)	0.997	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	14.4 %(w/v) PEG 8000, 160mM calcium acetate, 20% (v/v) glycerol, 80mM sodium cacodylate / HCl pH 6.5