7CYZ

The structure of human ORP3 OSBP-related domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97852
Spacegroup name	P 31 2 1
Unit cell lengths	95.336, 95.336, 185.251
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.282 - 2.100
R-factor	0.1754
Rwork	0.173
R-free	0.21920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ic4
RMSD bond length	0.009
RMSD bond angle	1.199
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.090	5.700	2.100
Rmerge	0.074	0.050	0.717
Rmeas	0.078	0.053	0.760
Rpim	0.025	0.017	0.247
Number of reflections	57791	3122	2825
<I/σ(I)>	7.6
Completeness [%]	100.0	99.5	100
Redundancy	9.8	9.1	9.2
CC(1/2)		0.997	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289.15	0.08 M Cacodylate sodium pH 6.6, 4.2% PEG 8000, 0.16 M Magnesium acetate